How to separate the breeding and quarantine of plant quarantine?

Isolation method: First, different culture media should be prepared to separate and cultivate various pathogenic bacteria. Usually, fungi usually use potato glucose agar medium (PDA), and bacteria usually use beef broth medium (NA). Both kinds of media are universal media suitable for the growth of most pathogenic bacteria, but it is sometimes difficult to get the expected effect when separating and culturing a specific pathogen, so some selective media must be used.

Tissue isolation method is suitable for the separation of pathogenic fungi. After surface disinfection and cleaning, the sample material was cut into small pieces, gently placed on the surface of the culture medium plate, labeled and cultured for observation.

Dilution separation method is suitable for the separation of fungi, actinomycetes and spore-producing bacteria.

The line separation method is mainly suitable for the separation of bacteria. After disinfection, the surface of the sample should be broken, soaked in sterile water 10 ~ 20 minutes before marking.

Isolation and inspection of plant parasitic nematodes. First, the pathogenic nematodes were isolated from the host, soil or other carriers, and then identified.