High performance liquid chromatography mobile phase and stationary phase are what?

1, reversed-phase stationary phase: C18, C8

2, normal-phase stationary phase: silica gel, amino-bonded, cyano-bonded

3, mobile phase: methanol, acetonitrile, water

I. Shape of stationary phases

1, fully porous

Initially, the porous spheres made of silica gel, alumina, and diatomaceous earth, etc.

100μm Large particles, surface impregnated with fixative. The mass transfer rate was slow and the column efficiency was low.? Small particles below 10μm are now used.

2. Surface porous type (thin shell type beads) ?

30 ~ 40μm glass microspheres, the surface attached to a layer of porous silica gel with a thickness of 1 ~ 2μm. Small surface area, fast mass transfer rate, but low column capacity.

Two, mobile phase categories

1, according to the composition of the mobile phase: single-component and multi-component;

2, according to the polarity: polar, weak polarity, non-polar;

3, according to the way of use: fixed-component drenching and gradient drenching