Advantages and disadvantages of liquid amylase activity determination in koji by spectrophotometry and visual inspection

1, spectrophotometer method. Advantages: high accuracy. Spectrophotometer determines the change of substance concentration caused by amylase reaction by measuring the light absorption of specific wavelength, which makes the measurement result more accurate. Spectrophotometer has high sensitivity and can detect very small light absorption changes, so it can also get reliable measurement results for low concentration amylase samples. The repeatability is good. This method adopts instrument measurement, which reduces the possibility of human error and improves the repeatability of experimental results. Disadvantages: The equipment requirements are high, and it is necessary to use a special instrument-spectrophotometer for measurement, which may require high cost and technical operation. There are many times and steps, so using spectrophotometer requires a series of operation and measurement steps, which is complicated and takes a long time to complete an experiment.

2. Visual inspection. Advantages: simple and easy to operate. Visual inspection is a simple and intuitive method, which can judge the activity of amylase only by observing the color change produced by amylase reaction, and the operation is relatively simple. The cost is low, the visual inspection method does not need special instruments, the cost is low, and it can be carried out in a simple laboratory environment. Disadvantages: strong subjectivity, visual method depends on the subjective judgment of the experimenter, there may be human error, and the accuracy and repeatability of the results are limited. Low sensitivity. Compared with the spectrophotometer method, the visual method is weak in identifying subtle color changes, and it cannot measure low-concentration amylase samples.