Brief introduction of automatic biochemical analyzer

Automatic biochemical analyzer (ACA): biochemical analyzer: an instrument used to detect and analyze biochemical substances, which provides information basis for clinical diagnosis, treatment, prognosis and health status.

Optical system: it is the key part of ACA. The old ACA system used tungsten halogen lamp, lens, color filter and photovoltaic module. The optical part of the new ACA system has been greatly improved. The beam splitting system of ACA can be divided into front beam splitting and rear beam splitting due to different light levels. Advanced optical components use a set of lenses between the light source and the cuvette, and the light projected by the original light source lamp is changed into the speed of light through the cuvette (different from the traditional wedge beam), so that even if the cuvette is smaller, the spot beam can pass through. Compared with the traditional method, it can save 40-60% reagent consumption. After passing through the cuvette, the spot beam is reduced to the original beam by this set of reducing lenses (wide aberration correction system) and divided into several fixed wavelengths (about 10 wavelength) by grating. The optical/digital signal direct conversion technology is adopted, that is, the optical signal in the optical path is directly converted into a digital signal. The interference of electromagnetic waves to signals and the attenuation during signal transmission are completely eliminated. At the same time, optical fiber is used in the signal transmission process, so that the signal is not attenuated and the test accuracy is improved by nearly 100 times. The closed combination of optical path system makes the optical path need no maintenance, accurate beam splitting and long service life.

Constant temperature system: Because the temperature has a great influence on the reaction results during biochemical reaction, the sensitivity and accuracy of constant temperature system directly affect the measurement results. Early biochemical instruments used air bath method, and later developed into dry bath with constant temperature liquid circulation and indirect heating, which combined the advantages of dry air bath and water bath. Its principle is to design a constant temperature tank around the colorimetric cup and add a stable constant temperature liquid, which is odorless, pollution-free, non-evaporating and non-deteriorating. Constant temperature liquid has large capacity, good thermal stability and uniformity. The cuvette is not in direct contact with constant temperature liquid, which overcomes the characteristics of easy pollution of constant temperature in water bath and uneven and unstable air bath.

Sample reaction stirring technology and probe technology: traditional reaction stirring technology adopts magnetic beads and eddy current stirring. At present, the popular mixing technology is a mixing unit composed of several groups of mixing rods, which imitates the manual cleaning process. When the first set of stirring rods stirs the sample/reagent or mixed solution, the second set of stirring rods simultaneously performs high-speed and efficient cleaning, and the third set of stirring rods simultaneously performs warm water cleaning and air drying. In the design of a single stirring rod, a new type of spiral high-speed rotation stirring is adopted, and the rotation direction is opposite to the spiral direction, which increases the stirring intensity, prevents the stirring liquid from foaming and reduces the light scattering of microbubbles. Both the reagent and the sample probe are based on the early capacitive sensing principle, but they are slightly improved, adding the alarm of blood clots and protein clots. According to the retest results of alarm level, the sampling error is reduced and the reliability of test results is improved. The detection times of large-scale biochemical instruments are above 1000 per hour, so it is very important to automatically recheck them. Subjective evaluation and manual re-examination of test results can no longer meet the clinical needs.

Other aspects: barcode identification and computer login of reagents and samples. Due to the lack of bar code recognition function, early biochemical instruments have more chances of making mistakes. In recent years, both imported and domestic biochemical instruments have adopted bar code detection. The application of this technology in biochemical instruments provides technical support for the development of high-speed ACA, and also makes the instrument quite supportive. Software development is simple and easy, so bar code detection is the basis of instrument intelligence. Open reagent, as an important factor in hospital selection, whether the instrument supports open reagent is very important. After the reagent is opened, hospitals and scientific research units can choose their own reagent suppliers, and have greater freedom in terms of measurement price, reliability of low-level instrument results, and validity period of reagents. Ion-selective electrode analyzer (ISE), the electrolyte index of human serum and urine is very important. The hospital can save money by adding ACA system to ISE.

Automatic analyzer is to automatically run all or part of the steps of sampling, mixing, warm bath (37℃) detection, result calculation, judgment, display and print results, cleaning and so on in the original manual operation process. At present, biochemical tests have basically realized automatic analysis, and there are also fully automatic biochemical analysis systems specially designed for large or super-large clinical laboratories and commercial laboratories, which can be configured at will according to the detection amount of laboratories.

Whether it is a modular automatic biochemical analyzer with the fastest running speed (9600Test/h) or an original manual photoelectric colorimeter for color comparison, its principle is to use absorption spectrometry in spectral technology. Is that most basic core of biochemical instrument. ◆ Technology-leading optical path reaction system

◆ Efficient and worry-free automatic flushing system

◆ Intelligent and sensitive liquid level detection system

◆ Simple and fast software operating system

◆ Timely and thoughtful national service network:

Detection speed: 300 times/hour (excluding ion detection)

The instrument has the power-on self-test function, and when the self-test is abnormal, a fault prompt is displayed;

The instrument is operated on the Windows platform, with an all-Chinese system and a reaction monitoring screen, and the operator can know the running status of the instrument at any time.

Optional working mode: You can arrange the examination in patient order or project order.

Inspection method: single and double wavelength method, single and double reagent method is optional.

Analysis mode: absorption spectrophotometry, endpoint method, two-point method, kinetics method, standard curve method, factor method and control tube method;

The instrument is open to reagents, and the project parameters can be preset according to the requirements of different reagents, and stored in the instrument after calibration for clinical inspection.

The instrument has the function of inserting emergency samples, and the inspection of emergency samples is given priority;

The instrument has the functions of sample pre-dilution and automatic re-inspection. When the test result of the sample is superlinear, the instrument can arrange pre-dilution and re-test.

The instrument has indoor quality control function, which can realize real-time monitoring of on-site inspection results;

The instrument has power-off protection function: after the power supply is restored, the original running program can be continued.

The reagent tank has 24-hour uninterrupted low-temperature refrigeration function, and the temperature in the tank is 2℃ ~10℃;

Instrument output: project parameters, calibration curve, dynamic response curve, absorbance, concentration, enzyme activity, judgment result of normal reference value, test data and quality control chart.

Sample system

Sample library: there are 65 sample positions, which can hold all kinds of original blood collection tubes, test tubes and micro sample cups. And there are two sample plates which can be used interchangeably.

Sample size: 2.5 ul ~ 40 ul, determined when the project is compiled, and the instrument automatically samples, increasing by 0.05ul.

Sample needle: it has automatic liquid level detection and anti-collision safety protection functions.

Cleaning of the sample needle: In addition to cleaning the inner and outer surfaces of the sample needle with special cleaning solution, the outer surface of the sample needle should also be cleaned with warm distilled water and dried with hot air.

operating system

Software operating system: Windows XP

Data processing: there is no time and storage limit for storing and outputting various test data and charts (including the calculation results of the project).

Advantages of operating software:

Chinese online help operation guide;

Full open test parameter setting;

Real-time response curve, full detection;

At the same time, the primary and secondary wavelengths are used to detect and eliminate interference, and the most accurate detection results are obtained;

Real-time diagnosis of reagent quality information, sample analysis results and the state of each unit of the system;

Unique user management password, assigning user's operation authority;

Sample test data editing. There are a variety of report styles for users to choose from.

Calibration and quality control:

Calibration method: Various calibration methods, single point, two points and multiple points (linear regression method, semi-logarithm method, total logarithm method, quadratic curve, cubic curve and four-parameter method).

Calibration period: set at any time according to user's needs.

Quality control software: three-level quality control; Equipped with L-J quality control chart, westguard, multi-rule, -R and other quality control rules.

Display: 17# LCD.

Printing function: Chinese printing content is optional, and remote report printing is supported.

System interface: RS-232 standard interface. Parameter list