What is the difference between gene mutation primer design and common pcr primer design?

According to Baidu Education, the difference lies in their different uses and application scenarios.

1. The main goal of common PCR primer design is to ensure that the sequence of primer and template DNA is complementary, so that the target fragment can be effectively amplified in PCR reaction. Therefore, for ordinary PCR primers, the key to design is to select the sequence complementary to the specific region of template DNA. Generally, the length of primers should be between 15-30bp, the difference between upstream and downstream primers should not be too big, and the primers themselves should not have the complementarity of four consecutive bases.

2. The design of gene mutation primers is to purposefully replace bases at specific positions on the basis of known gene sequences, usually to study the key functional domains of a gene, even the functions of specific amino acid sites. Therefore, the key to design mutant primers is to mutate as few bases as possible to change amino acids. For example, if it is necessary to mutate the codon GCC encoding Ala into GCC (that is, without changing the amino acid), it is necessary to change 1 base.