What are the factors that affect pathological Pap staining?

1, dye concentration: toluidine blue and hematoxylin are usually used in pathological Pap staining, and the dye concentration will affect the intensity and clarity of dyeing. If the dye concentration is too high or too low, it will lead to unsatisfactory dyeing effect.

2. Staining time: The time of pathological Pap staining is an important factor to control the dyeing effect. Long-term staining leads to over-staining, which makes the nucleus and cytoplasm of the tissue too dark and difficult to distinguish. However, too short staining time will lead to insufficient staining and affect observation and diagnosis.