What are the methods of microbial inoculation?

First, the plate line inoculation method (separation culture method)

This method is a common technique for isolating and culturing bacteria. Its purpose is to make the culture mixed with various bacteria or different bacteria (pathogenic bacteria and non-pathogenic bacteria) in the sample grow dispersedly to form a single colony or separate a single strain, which is convenient for identification. There are many methods of plate marking inoculation, among which subsection marking method and curve marking method are commonly used. ?

Second, oblique inoculation method?

This method is mainly used for transplanting pure bacteria, making them proliferate, and for identification or preservation of strains. Usually, a single colony is selected from a plate culture or a growing slant strain is transplanted into a slant culture medium. The inoculation steps are as follows (taking the strain transfer on the growth slope as an example)?

Third, the inverted culture method

This method is used to count bacteria and molds in feed. The method is to take the original sample or 1 ml properly diluted liquid (generally diluted by 10- 1~ 10-5), put it in a sterile dish with a diameter of 9 cm, and pour it into the culture medium that has been melted and cooled to about 50℃ for about13 ~/kloc. ?

Fourth, puncture inoculation method?

This method is mostly used for inoculation of disaccharide, semisolid, gelatin and other media, and the method is similar to slant inoculation. Pick a little colony or bacterial liquid with an inoculation needle and stab it from the center of the culture medium surface to the bottom of the tube (if it is iron trisaccharide and coated on the inclined plane), and then pull out the inoculation needle along the puncture line (note: those inoculated with semi-solid will not puncture to the end).

Verb (abbreviation of verb) liquid inoculation method

This method is mostly used in monosaccharide fermentation experiments, and the inoculation method is basically the same as the slant inoculation method. Holding the culture medium and strain tube in the left hand, holding the inoculation ring and cotton plug in the right hand, inoculating the selected colony or bacterial liquid into the liquid culture medium.

Extended data:

Inoculation of bacteria should use an inoculation needle (ring) to take bacterial samples for inoculation. Is the inoculation ring and needle platinum wire or not? Made of alloy wire, it can also be replaced by electric furnace wire, because it is resistant to high temperature, fast in heat dissipation, and convenient for flame sterilization before and after inoculation (the whole inoculation ring burns red to achieve sterilization purpose).

When using the inoculation ring, it is more convenient to hold the pen in the right hand and cooperate with the culture medium in the left hand. The inoculation procedure can be divided into five procedures: inoculation ring sterilization → slight cooling → taking bacterial samples → inoculation (including: opening or plugging, inoculation marking, capping or plugging) → inoculation ring sterilization. Different media have different inoculation methods.

References:

Baidu encyclopedia-vaccination