Traditional Culture Encyclopedia - Traditional stories - Who can summarize the steps of extraction, separation and purification of microbial DNA in simple language?
Who can summarize the steps of extraction, separation and purification of microbial DNA in simple language?
Ultrasonically crushing cells, adding sds and pk, centrifuging to obtain supernatant, adding equal volume of phenol, centrifuging to obtain supernatant, adding equal volume of chloroform, centrifuging to obtain supernatant, adding 2-3 times of ethyl at -20℃ overnight, centrifuging to obtain supernatant (dna precipitation), and fully drying. Electrophoresis can be performed for further purification.
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