What are the main types of nucleic acid extraction or purification technologies?

There are three main types of nucleic acid extraction or purification techniques:

1, the traditional method (complicated operation and long time-consuming)

2. Centrifugal column method (kit)

3. Magnetic bead method (magnetic beads can be purchased separately or the finished kit)

Among them, magnetic bead method can realize automatic operation and is the mainstream method at present.

Nucleic acid extraction is applied in many fields such as disease control center, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial detection, food safety detection, animal husbandry and molecular biology research.

Extended data:

Matters needing attention in nucleic acid extraction:

1. Installation environment of the instrument: normal atmospheric pressure (altitude should be lower than 3000m), temperature 20-35℃, typical service temperature 25℃, relative humidity 10%-80%, and air at or below 35℃ flows in smoothly.

2. Avoid placing instruments near heat sources, such as electric heaters; At the same time, in order to prevent short circuit of electronic components, water or other liquids should be avoided from splashing into them.

3. Both the air inlet and the air outlet are located at the back of the instrument to prevent dust or fibers from gathering at the air inlet and keep the air duct unblocked.

4. The distance between the nucleic acid extractor and other vertical surfaces is at least 65438±00cm.

5. Instrument grounding: In order to avoid electric shock, the input power cord of the instrument must be grounded.

6. Stay away from live circuits: The operator shall not disassemble the instrument without authorization, and the replacement of parts or internal adjustment must be completed by certified professional maintenance personnel. Do not replace components when the power supply is connected.