How to collect hematopoietic stem cells from bone marrow?

Bone marrow hematopoietic stem cells are a method to collect hematopoietic stem cells directly from red bone marrow. Red bone marrow is the main place where hematopoietic stem cells exist. Because the blood in the bone marrow cavity is also extracted at the same time, in order to prevent the donor from losing too many red blood cells and causing anemia or hypotension. Before donating bone marrow, donors need to prepare their own blood: that is, 600 ml of their own blood is gradually collected a few weeks before donation, stored in vitro, and input during surgery. The collection of bone marrow hematopoietic stem cells needs to be operated under general anesthesia or epidural anesthesia. During the operation, after anesthesia, the puncture needle was inserted into the ilium on both sides of the hip to extract bone marrow cells. With the need of extracting bone marrow cells, iliac bone can be punctured at multiple points. The amount of bone marrow extracted depends on the patient's weight, and about 500~ 1000 ml of bone marrow fluid needs to be extracted. At the same time, transfuse raw blood into the body to avoid anemia. The collection of bone marrow hematopoietic stem cells will not cut the skin, but will leave several puncture pinholes on the skin surface. After the operation, bleeding at the wound must be prevented. The injured part may feel pain and can be treated according to the symptoms. Most donors also feel low back pain. In addition, some blood donors will feel sick and vomit because of the anesthetic. Because the number of white blood cells in the body is temporarily low, there may be a slight fever. These conditions can also be treated by doctors according to symptoms.