The method of executing rats

The following methods are applicable to small animals such as rats and mice.

(1) Spine dislocation method: the animal's cervical vertebra is dislocated and the spinal cord is disconnected, resulting in the animal's death. The left thumb and forefinger pressed the mouse's head down hard, and the right hand grabbed the mouse's tail and pulled it back hard, and the mouse died immediately. This is the most commonly used method.

(2) Acute massive blood loss: eyeball can be removed, resulting in massive blood loss and death.

(3) Strike method: Grasp the tail of the mouse with the right hand, lift it and strike its head hard, and the mouse will die immediately after convulsion. You can also kill a mouse by hitting it on the head with a mallet.

(4) decapitation method: When decapitation is given to mice, the mouse scapula can be clamped and fixed with the thumb and forefinger of the left hand. Take scissors in your right hand and cut your head quickly. When decapitating a mouse, the experimenter should wear cotton gloves, hold the mouse's head with his right hand, hold its back with his left hand to expose his neck, and the assistant will cut off the mouse's head with scissors.

(5) Cotton balls soaked in ether or chloroform can be sealed with small animals in a glass container for anesthesia.