What are the factors that affect the extraction rate of berberine?

(1) Different extraction processes have effects on the extraction rate of berberine. There are many extraction methods of berberine, such as impregnation, decocting method, percolation, common reflux extraction, continuous reflux extraction, ultrasonic extraction, supercritical extraction and so on.

(2) Different extraction solvents, extraction temperature, extraction time and solid-liquid ratio will affect the extraction rate of berberine;

(3) Extraction from plants, the factors affecting the extraction rate of berberine are raw material crushing degree, soaking time, one-time feeding amount, reflux time, temperature, pressure and so on.

Extended data

(1) The optimum conditions for extracting berberine from Rhizoma Coptidis are as follows: soaking in 5% sulfuric acid solution for 24 hours, ultrasonic treatment with 20KH2 for 30 minutes, and filtering; The residue is repeatedly extracted once; Mixing filtrates, purifying, concentrating, and crystallizing.

(2) Extraction rate and efficiency of berberine from Cortex Phellodendri. The extraction process of berberine from Phellodendron amurense was optimized by combining the traditional alkali extraction method with enzymolysis-assisted extraction.

Taking the extraction rate of berberine as the evaluation index, the effects of cellulase concentration, enzymolysis time, material-liquid ratio and extraction time on the extraction efficiency were investigated. On the basis of single factor experiment, the function and interaction of each factor are analyzed through orthogonal experiment.

The optimum technological conditions of enzymatic alkali extraction of berberine from Cortex Phellodendri are as follows: cellulase concentration 15‰, enzymolysis time 1h, solid-liquid ratio 1∶ 7, and extraction time of 7 h. Under these conditions, the extraction rate of berberine can reach 8.99%.