Preparation of anti-unique antibody vaccine

There are two ways to prepare anti-id antibodies: by traditional immune serum preparation and by monoclonal antibody preparation. However, whichever way the anti-Id antibody is prepared, it must be absorbed on an agarose 4B affinity chromatography column crosslinked with normal immunoglobulin (homologous to the antibody used for immunization) and purified on an agarose 4B affinity chromatography column crosslinked with purified unique antibody (i.e., the immunizing antibody), and then, finally, the purified anti-Id antibody must be comprehensively identified. As far as these two preparation methods are concerned, they each have their own characteristics: polyclonal anti-Id antibodies are simple to prepare, and the amount of internal image anti-Id antibodies (i.e., antibodies directed against the unique type-determining clusters of the antibody-binding site) obtained is low; monoclonal anti-Id antibodies are more complicated to prepare, but once the internal image anti-Id antibodies are obtained, their yield is unlimited.