How to choose bacterial inoculation method

1. streaking method: it is mainly used to separate strains. A small amount of substances to be separated are obtained from the inoculation ring and streaked continuously on the surface of sterile plate, so that the number of microbial cells decreases and gradually disperses with the increase of streaking times.

2. Dumping method: mainly used for counting the total number of colonies. Suck 1ml bacterial liquid into the plate, pour it into the bacterial culture medium, gently rotate the plate to mix the bacterial liquid and the culture medium evenly, invert it after cold suspicion, and cultivate it at a suitable temperature.

3. Inclined inoculation method: mainly used for strain preservation. Insert the inoculation ring or needle into the strain tube to select colonies for transplantation. After inoculation, the culture holes were sterilized by flame and cultured at 37 degrees Celsius.

4. Inoculation method of liquid culture medium: It is mainly used in bacteria-liquid turbidimetry experiment, in which colonies or specimens are picked up by a sterile inoculation ring and gently ground at the junction of the inner wall of the test tube and the liquid surface, so that bacteria are evenly dispersed in the liquid culture medium.

5. Spiral inoculation method: rapid bacterial inoculation can be carried out under dilution conditions. The reduced sample volume is automatically distributed on the surface of the rotating medium in the form of archimedean spiral. The concentration of bacterial liquid can be calculated by dividing the number of colonies in a certain area of the Petri dish by the amount of samples injected in the same area.