About the detection of Salmonella

Salmonella belongs to the family of intestinal bacteria, including those that cause food poisoning, gastroenteritis, typhoid fever and paratyphoid fever. It can cause food-borne diseases and has become the most common cause of food poisoning in recent years. Salmonella enteritis infection usually comes from dairy products, poultry products and meat products; Chicken and eggs are particularly high-risk foods. The symptoms of salmonella infection are usually gastrointestinal problems, including nausea, abdominal cramps, vomiting and diarrhea, which usually last up to 7 days. If people with low immunity do not take antibiotics in time, salmonella infection will lead to life-threatening. Salmonella test piece (FilmplateTM Salmonella BS205) contains selective medium, color indicator of octyl esterase unique to Salmonella and polymer water-absorbing gel. Using the patented technology of microbial test piece, a disposable rapid detection product was made. One-step culture15 ~ 24 hours can confirm whether there is salmonella, which is very suitable for inspection departments and food enterprises at all levels. This product is suitable for rapid detection of meat and meat products, eggs and egg products, cold drinks, etc.

Operation method of Salmonella Fiona Fang test piece:

1. Sample treatment: Take 25mL(g) sample and put it into a sampling tank or homogenization cup filled with 225mL diluted sterile phosphate buffer (or physiological saline) to prepare a sample homogenate of 1: 10.

2. Inoculation: Put the test piece of Salmonella (BS205) on a flat experimental platform, uncover the upper membrane, suck the 1ml sample with a sterile pipette, slowly and evenly drop it on the paper, then slowly cover the upper membrane and let it stand for about 10s to solidify the culture medium, and inoculate two pieces for each sample. At the same time, a blank negative control was made.

3. Culture: Stack the test pieces together and put them back into the original self-sealing bag for sealing. The transparent surface is horizontally placed in a constant temperature incubator, and the number of stacked pieces shall not exceed 12. The culture temperature is 36℃ 65438 0℃ and the culture time is 65438±05 ~ 24h.

Interpretation of results:

Observing the test piece, it was found that the purple colony was Salmonella. The blue colonies are other flora. For samples with positive colonies, it is best to use other more reliable methods for verification. If there are no conditions, at least another sample should be taken for repeated inspection.