Answer questions about bioengineering. Bioengineering has a broad application prospect. The following is the production process of interferon. (1) comes from human lymph.

(1) When the target gene (interferon gene) is obtained, it needs to be cut by restriction endonuclease.

(2) When constructing gene expression vector, DNA ligase is needed to combine the target gene with the vector to form recombinant DNA molecules. Engineering bacteria refers to the strain cell line that can express foreign genes efficiently through genetic engineering, so yeast is called engineering bacteria in this genetic engineering.

(3) As a vector, it must meet the following conditions: (1) it must have the cleavage site of restriction endonuclease; ② There should be marker genes (such as resistance genes) to facilitate the screening of recombinant; ③ It can stably exist and replicate in the host cell; ④ It is safe, harmless to recipient cells and easy to be separated from donor cells. Natural plasmids cannot be directly used as vectors, and the plasmids used in genetic engineering are all artificially modified on the basis of natural plasmids.

(4) It is necessary to screen yeast that can successfully express interferon gene. The engineering technology that can produce interferon in large quantities by using transgenic yeast is called genetic engineering.

So the answer: (1) restriction endonuclease? Target gene

(2)DNA ligase engineering bacteria

(3) Activity-based costing

(4) screening? gene engineering