What is the principle of antibody purification?

The principle of all purification is to keep what I want and remove what I don't want. The most ideal is the purity 100% and the yield 100%.

But everyone knows it's impossible.

Antibody purification is mainly based on types and requirements. For example, monoclonal antibodies are pure enough after passing through protein A or protein G columns. If it is a secondary antibody, it can be purified with immunoglobulin. Polypeptide antibodies can also be purified with polypeptide antigens. If it is a polyclonal antibody against cytokines, because cytokines are too expensive, it can also be purified by protein A or protein G column or simply by ammonium sulfate.

You can find some domestic antibody companies, find a similar antibody and ask them how to purify it.

Besides, depending on what you do, it requires different antibody purity. The other is the loss of antibody quantity and activity during purification.