Traditional Culture Encyclopedia - Traditional festivals - Why should we cultivate single cells to ensure the purity of antibodies?
Why should we cultivate single cells to ensure the purity of antibodies?
When you say single cell, you mean monoclonal cell.
The screened monoclonal cells can indeed produce specific antibodies.
In order to prevent the antigen itself from having multiple antigenic determinants, resulting in the same antigen producing many different antibodies, monoclonal proliferation is usually used.
If the antigen can only produce one antibody, in theory, there is no need for post-screening. However, if it is necessary to ensure the stability of antibody expression, it is often necessary to find the best antibody secreting cells through screening.
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