Advantages and disadvantages of immobilized enzyme

Advantages and disadvantages of immobilized enzyme:

First, advantages

1, can be used for many times, and has high stability and high unit enzyme yield.

2. It is easy to separate from substrate and product, and there is no enzyme residue, which simplifies the purification process of the product, improves the product yield and has better quality.

3. The catalytic reaction conditions are easy to control, and can be packed in columns for continuous reaction, which is suitable for automatic production, saves labor and reduces the occupied area of the reactor.

Second, shortcomings.

1. When many enzymes are immobilized, it is necessary to use toxic chemical reagents to combine enzymes with carriers. If these reagents remain in food, it will have a great impact on human health.

2. In the process of continuous operation, some microorganisms often multiply in the reaction system, which uses the nutrients of food for growth and metabolism and pollutes food.

3. After immobilization, the enzyme activity was lost.

4. The production cost increases and the initial investment of the factory is large.

5. It can only be used for soluble substrates, which is more suitable for small molecular substrates and not suitable for macromolecular substrates.

6. Compared with intact bacteria, it is not suitable for multi-enzyme reactions, especially those requiring cofactors.

7. Intracellular enzymes must go through an enzyme separation procedure.

Science popularization of immobilized enzyme

Immobilized enzyme is a new technology developed in 1960' s, which refers to an enzyme that can play a catalytic role in a certain space and can be used repeatedly and continuously. Usually, the enzyme-catalyzed reaction is carried out in aqueous solution, while the immobilized enzyme is treated by physical or chemical methods to make it insoluble in water, but it still has enzyme activity.