Microbiological examination procedure for Mycobacterium tuberculosis

(1) Collection of diseased materials: take the tissues, liver, lung, spleen and other body fluids of diseased livestock and poultry, and the secretions and exudates of local diseases.

(2) Microscopic examination: Gram staining was performed on the smear of primary disease materials, and the microscopic examination should be Gram-negative. Dyed with dyes such as Indian ink, you can see clear capsules.

(3) Culture: Inoculate blood agar and Mai Kangkai agar culture medium at the same time, culture at 37℃ for 24 hours, observe bacterial growth, colony characteristics and hemolysis, and do staining and microscopic examination.

(4) Biochemical test: Pasteurella multocida can decompose glucose, fructose, lactose, sucrose and mannose within 48h, producing acid without producing gas.

The main tasks of microbiology:

The role of 1 in natural material circulation.

2, air and water purification, sewage treatment.

3. Industrial and agricultural production: bacteria, metabolites and metabolic activities.

4. Contribution to life science.

Extended data:

An organism that can only be seen with the help of a microscope. Microorganisms are distributed in air, land, rivers and oceans. There are many kinds of microorganisms in people, livestock and plants, some of which are pathogenic and harmful to human beings, but there are also many microorganisms that are beneficial to human beings.

For example, the koji of wine-making is composed of some microorganisms, and the whole fermentation industry is inseparable from microorganisms. Microorganisms existing in soil are called soil microorganisms, and there are many kinds, which can be roughly divided into bacteria, fungi, actinomycetes and so on. Some algae and nematodes can also be classified as soil microorganisms.