How are monoclonal antibodies prepared?

Monoclonal antibody is a highly homologous antibody produced by a single B cell clone, which only targets a specific epitope. Usually prepared by hybridoma technology. Hybridoma antibody technology is based on cell fusion technology, which fuses sensitized B cells with specific antibody secretion ability and myeloma cells with unlimited reproduction ability into B cell hybridoma.

A single hybridoma cell with this characteristic can be cultured into a cell population, and a specific antibody against the epitope, that is, a monoclonal antibody, can be prepared.

Monoclonal antibodies are produced by artificially prepared hybridoma cells, which are fused by antigen-activated B cells and myeloma cells. The monoclonal antibody has high purity, high sensitivity, strong specificity, less cross reaction and low preparation cost. Disadvantages: there are certain requirements for technology, and it is easy to lose epitopes by chemical treatment of antigens.