Traditional separation, purification and culture

There are many factors that affect the morphology of bacteria, such as culture medium composition and culture time. The morphology of bacteria is different in different growth stages, especially in recession. So the bacteria you see may be in different growth stages, the state of the culture medium is also different, and the shape should not be exactly the same. If you want to observe the bacteria with the same morphology, you'd better choose the stable or several bacteria solutions with the same liquid culture time for dyeing observation, and maybe you can see the same morphology.