What are the advantages of digital PCR?

Digital PCR is an absolute quantitative technique for nucleic acid molecules.

Digital PCR is an enzymatic synthesis reaction dependent on DNA polymerase in the presence of template DNA, primers (known sequences at both ends of template fragment) and four deoxynucleotides. The specificity of amplification depends on the specific combination of primer and template DNA. Compared with QCPR, BIOG digital qPCR can directly count the number of DNA molecules, which is an absolute quantification of the initial sample.

Extended data:

3D digital PCR is based on nanofluid chip for detection. Through one test, the sample is divided into thousands of separate PCR, and some of the test results are positive and some are negative, and then the absolute number of molecules can be counted without calibration. At present, 20,000 droplets (samples) of 0.8nL can be detected, and the target position can be accurately and quantitatively studied.