Cultivation technology of biological microorganisms in senior high school

There are different methods for microbial inoculation according to different experimental purposes, but most of them are labeled after inoculation. I wonder what experiment the teacher wants you to do? The following content will be clearer if you have done experiments!

Isolation of single colony: scribing is the most commonly used method?

Timing of use: pure culture of microorganisms

Separate multiple colonies: for example, after blue and white selection. I want to choose a white strain with a gene inserted into the bacterial plasmid.

First, we will pick out the desired white colonies, and then inoculate small areas and mark them, as shown below, marking the strains 1 ~ 9. After the next day's culture, we will take some bacteria for PCR confirmation, and then we can choose them for strain preservation, such as strain 6.

It is very important to mark the strains because they all look the same and can't be distinguished at all.