How do I prepare my own antibodies? How to test the purity of antibodies?

It is to inject your target antigen to the animal, after a period of immunization, the animal can produce antibodies, you can collect blood, separate the serum, and there are antibodies in the serum.

Antibody detection can be as simple as a coagulation test or a precipitation test to determine if there are any antibodies, and if there are, you can also determine the purity.

To determine the purity of the cross-test can be used, that is, with your prepared serum to react with different antigens, to see if there is binding ability, if there is a non-can be combined with other antigens, may be your antigens are contaminated, or the animal was exposed to other antigens.

The purity test can also be done by physicochemical methods, which are more complicated, and there are a lot of methods now, but not many of them are really practical. Keep in touch if you need more information