How is an electrophoresis experiment performed?

Electrophoresis experiment is a common biochemical experimental method, usually used for the separation and detection of biological molecules (such as proteins, DNA, RNA, etc.) or particles of the nature and size of the charge. The following are the general electrophoresis experiment steps:

1. Preparation of electrophoresis buffer: according to the experimental needs, prepare the appropriate pH buffer to maintain the appropriate ionic strength and pH conditions, usually using Tris buffer or phosphate buffer.

2. Prepare the electrophoresis tank: Install the electrophoresis tank on a horizontal table, fill enough electrophoresis buffer, and make sure the electrodes and electrophoresis tank are connected correctly.

3. Prepare the sample for electrophoresis: mix the sample of biomolecules or particles to be detected with the appropriate amount of electrophoresis buffer, usually some electrophoresis buffer needs to be added for dilution.

4. Load the sample: add the prepared sample into the sample tank of the electrophoresis tank, usually need to use a micropipette or a pipette to operate.

5. Start electrophoresis: Connect the electrophoresis tank to the power supply, set the appropriate voltage and run time, and start the electrophoresis experiment. Usually start at a lower voltage and gradually increase the voltage to avoid thermal distortion of the sample.

6. Result analysis: According to the time and voltage of electrophoresis, the biomolecules or particles to be detected will move to different positions in the gel, which will be analyzed and interpreted according to the speed and distance moved.

It should be noted that different types of electrophoresis experiments will have some special steps and requirements, such as protein electrophoresis, DNA electrophoresis, agarose gel electrophoresis, etc., which should be performed according to the lab guide.