Answer the following questions about microorganisms. Before the clinical trial of antibiotics, it is sometimes necessary to do bacterial resistance test. The first thing to do is to obtain a small amo

Answer the following questions about microorganisms. Before the clinical trial of antibiotics, it is sometimes necessary to do bacterial resistance test. The first thing to do is to obtain a small amount from the patient. (1) Isolation of colonies commonly used dilution smear plate method or line smear method.

(2) In the expanded culture of pathogenic bacteria in the choice of liquid medium.

(3) In the process of resistance testing, if there is a transparent circle around the colony, it means that the antibiotic can inhibit the bacteria; the size of the transparent circle indicates the antibacterial strength of the antibiotic. Accordingly, we can determine the antibacterial strength of A, C, B; D of the transparent circle appeared in a colony, indicating that the colony of this antibiotic is resistant.

(4) According to the analysis of (3), the experimental results of antibiotic A is the best effect on the pathogenic bacteria.

(5) The inoculated whole nutrient medium is used as a control, i.e., the inoculated beef paste peptone medium can be used to determine whether the selection medium has played a selective role.

The answers are:

(1) Line method Dilution coating method

(2) Liquid

(3) Sensitive Insensitive? Resistant bacteria

(4) A

(5) C