Advantages and disadvantages of immobilized enzyme by embedding method

1, the method is simple, the cells are agglomerated with monomers or polymers, and the cells are embedded in the formed polymers.

2. The conditions are mild, and different polymer carriers, different embedding systems and conditions can be selected to maintain the enzyme catalytic activity of cells.

3. The battery is not easy to leak and has good stability.

4. It has high cell capacity, and the cell content in the polymer can reach 40%-70%.

disadvantaged

Because the immobilized cell technology is not mature enough, there are still some shortcomings in the toxicity and some properties of immobilized cell embedding materials, the strength of immobilized cells and the absence of miscellaneous bacteria. Immobilized cell technology is mainly used in sewage treatment. In order to better apply immobilization technology to the production of food and medicine, it is necessary to develop more and better non-toxic and cheap embedding materials and constantly explore ways to improve the strength of immobilized cells.

Definition of embedding method: the method of embedding enzymes, cells or protoplasts in various porous carriers to immobilize them.

Classification: According to the different carrier materials and methods, it can be divided into gel embedding method (grid embedding method) and semi-permeable membrane embedding method (microcapsule embedding method).

After enzyme immobilization, the stability is generally improved, and it is easy to separate from the reaction system, easy to control and can be reused. It is convenient for transportation and storage, which is beneficial to automatic production, but the activity is reduced, the application scope is narrowed, and there is still room for development of technology. Immobilized enzyme is an enzyme application technology developed in recent ten years, which has attractive application prospects in industrial production, chemical analysis and medicine.