Hepatitis B five enzyme-linked immunosorbent assay

1) HBsAg double antibody sandwich method, carrier (mostly plastic) adsorption of hepatitis B surface antibody and serum HBsAg and enzyme conjugate (antibody) to form antibody-antigen-enzyme conjugate complex, enzyme complex decomposition of the substrate (hydrogen donor is mostly tetramethylbenzidine) to generate a blue complex.2) HBsAb double antigen sandwich method, carrier adsorption of hepatitis B surface antigen and serum HBsab and add enzyme conjugate (enzyme antigen) to form antigen-antibody-enzyme antigen complex, the complex decompose the substrate to produce blue complex. 3) HBeAg double antibody sandwich method, the principle is the same as HBsAg. 4) HBeAb competition method, carrier adsorption of antigen and serum HBeAb binding to form a complex, and then add the enzyme conjugate (antibody), due to the carrier does not have redundant antigen-binding site, enzyme conjugate In the free state, in the plate washing, the enzyme conjugate is washed away, can not decompose the substrate and no color reaction, colorless is a positive result; if the serum does not have HBeAb, the carrier antigen and the enzyme conjugate can break down the substrate to show the L-blue color, the result is negative.5) HBcAb competition method with HBeAb

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