What are the characteristics of monoclonal antibodies compared with conventional antibodies?

Compared with conventional serum antibodies, monoclonal antibodies have the greatest advantages of strong specificity, high sensitivity and large-scale preparation, so monoclonal antibodies have the characteristics of strong specificity, high sensitivity, high purity and high yield compared with conventional antibodies.

Monoclonal antibodies are highly unified in physical and chemical properties, single in biological activity, strong in specificity of binding with antigens, convenient for manual operation and quality control, and easy in source, so they have been welcomed and valued since they came out. Monoclonal antibodies play an important role in diagnosing diseases, judging prognosis, preventing and treating diseases and studying disease mechanisms.

Extended data:

Precautions:

Technical mistakes often lead to the failure of integration. For example, no immune response was found in donor lymphocytes. This is doomed to failure.

The biggest failure reason of fusion test is pollution, and the key to successful fusion is to provide clean environment and suitable aseptic operation technology.

In the process of adoption, the HAT scheme and the complete 1640 scheme were cancelled and replaced by 10%FCS- 1640 scheme. At the same time, it was preserved in liquid nitrogen for cloning. During this period, each generation should check antibodies to prevent the variation and loss of antibody-producing cells.

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